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Problems with purifying antibodies from culture supernatant

3 Problems with purifying antibodies from culture supernatant [Pg.151]

1 Low concentration of antibody in culture supernatant compared with serum [Pg.151]

Concentration of culture supernatant using a hollow fibre device [Pg.151]

Peristaltic pumps of choice (e.g. Masterflex with size 17 pump head) [Pg.151]

Culture supernatant, preferably contained in a single flexible bag (e.g. Stedim) [Pg.151]

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Antibodies purified

Culture problems

Problems with)

Supernatant

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