Proanthocyanidins method performance

Rigaud, J. et ah. Micro method for the identification of proanthocyanidin using thiolysis monitored by high-performance liquid chromatography. J. Chromatogr. 540, 401, 1991. 

Lazarus, S.A. et al.. High-performance liquid chromatography/mass spectrometric analysis of proanthocyanidins in foods and beverages. Methods EnzymoL, 335, 46, 2001. 

Sephadex LH-20 (Pharmacia) is a support for gel chromatography and is widely used in isolation protocols for proanthocyanidins. It is composed of dextran molecules which are three-dimensionally cross-linked with hydroxypropyl groups [205]. Proanthocyanidins are separated by filtration (molecule sieve effect of the gel) and adsorption (hydrogen bonding). Retention increases primarely with increasing ( ) molecular weight and C4—>C6 linked procyanidins are more retarded than their C4—>C8 linked counterparts [206-207]. Sephadex LH-20 has first been used for sample clean up in the analysis of apple ciders by Lea and Timberlake [208]. So far, no pre-packed columns exist on the market. All methods described in the literature work with different column dimensions (see Tab. (6)). Standardization would help to make results more comparable. Some validation procedures have been performed for two protocols [185,209],... 

Lazarus SA, Hammerstone JF, Adamson GE, Schmitz HH (2001) High-performance liquid chromatography/mass spectrometry analysis of proanthocyanidins in food and beverages. In Lester P (ed) Method enzymol. Academic, New York... 

---