Principles of thermal enzyme sensors

When an enzymatic reaction gives rise to a variation in enthalpy (AH) in the presence of substrate (S), then the change in temperature (6) is a function of time (t) according to the expression [12]  

When the thermal sensor response reaches the steady state then 30/3t = 0, and the following relationship applies  

Numerical resolution of this equation gives the temperature profile at the interior of the enzymatic layer fixed to the thermistor. Theoretically it is now possible to find the temperature measured by the thermistor as a function of the different substrate concentrations in the sample medium, notably  

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