Principle of Thin-Layer Electrophoresis

An ordinary TLC plate is evenly sprayed with a suitable buffer solution. The substance mixture to be separated is applied to the moist layer as a spot or band and an electric field applied to the plate. The substances migrate at various speeds and in a direction depending on their 

A schematic representation of a separation is given in Fig. 55. The substances negatively charged at the pH used (1, 2, 3, 5) migrate towards the anode the positively charged (4, 6) towards the cathode. 

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