Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Primer extension by reverse transcriptase

Maxam-Gilbert load buffer 80% deionised formamide 10 mM NaOH 1 mM EDTA 0.02% xylene cyanol 0.02% bromophenol blue RT-X-dideoxynucleotide mix 2.5 mM (dNTP-dXTP) [Pg.219]

AMV reverse transcriptase (Life Science) tRNA carrier 5 mg/ml 5-end-labelled extension primer NaOAc 0.3 M (pH 6.0) [Pg.219]

Terminate the reaction by addition of 40 pi 0.3 M NaOAc (pH 6.0) and precipitate with 125 pi EtOH. [Pg.220]

Redissolve the dried pellet in Maxam-Gilbert load buffer. [Pg.220]


See other pages where Primer extension by reverse transcriptase is mentioned: [Pg.219]    [Pg.1144]   


SEARCH



Primer extension

Transcriptase

© 2024 chempedia.info