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Primed synthesis single stranded template

Primed single strands. A single stranded DNA can be converted into an efficient template for DNA synthesis by annealing a short... [Pg.13]

Currently the dideoxy method probably is the method of choice for sequencing DNA by primed synthesis methods and is generally applicable to any DNA that can be obtained in single-stranded form. In the last two years two important advances have been made which have essentially solved the problem of preparing the DNA template in single-stranded form and these are described in the following Section and in Chapter 4. The first method, which... [Pg.85]

Primed synthesis methods require that either the primer or the template DNA is in single-stranded form. Sometimes it is possible to physically separate the complementary strands from a duplex DNA but this is not always a straightforward procedure. The methods developed by Maxam and Gilbert (1977) (Chapter 5 of this volume), while eminently suitable for separating single... [Pg.124]

Only (c) would lead to DNA synthesis because (a) and (b) have no primer or open end to build on and (d) has no template extending beyond a free 3 -OH. Note Single-stranded linear DNA can be used as a template for DNA synthesis because it can prime synthesis through hairpin formation at its 3 end. [Pg.80]


See other pages where Primed synthesis single stranded template is mentioned: [Pg.144]    [Pg.4]    [Pg.5]    [Pg.14]    [Pg.16]    [Pg.24]    [Pg.62]    [Pg.89]    [Pg.122]    [Pg.209]    [Pg.237]    [Pg.345]    [Pg.235]    [Pg.237]    [Pg.378]    [Pg.13]    [Pg.27]    [Pg.30]    [Pg.85]    [Pg.107]    [Pg.288]    [Pg.355]    [Pg.153]    [Pg.32]    [Pg.177]    [Pg.436]    [Pg.158]    [Pg.271]    [Pg.322]    [Pg.454]    [Pg.322]    [Pg.25]    [Pg.91]    [Pg.989]    [Pg.31]    [Pg.222]    [Pg.411]    [Pg.381]    [Pg.98]   
See also in sourсe #XX -- [ Pg.13 , Pg.74 , Pg.152 ]




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Prime

Primed synthesis

Single synthesis

Single-strand

Single-stranded

Synthesis templated

Template synthesis

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