Primed synthesis single stranded template

Primed single strands. A single stranded DNA can be converted into an efficient template for DNA synthesis by annealing a short... 

Currently the dideoxy method probably is the method of choice for sequencing DNA by primed synthesis methods and is generally applicable to any DNA that can be obtained in single-stranded form. In the last two years two important advances have been made which have essentially solved the problem of preparing the DNA template in single-stranded form and these are described in the following Section and in Chapter 4. The first method, which... 

Primed synthesis methods require that either the primer or the template DNA is in single-stranded form. Sometimes it is possible to physically separate the complementary strands from a duplex DNA but this is not always a straightforward procedure. The methods developed by Maxam and Gilbert (1977) (Chapter 5 of this volume), while eminently suitable for separating single... 

Only (c) would lead to DNA synthesis because (a) and (b) have no primer or open end to build on and (d) has no template extending beyond a free 3 -OH. Note Single-stranded linear DNA can be used as a template for DNA synthesis because it can prime synthesis through hairpin formation at its 3 end. 

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