Proteolysis preventive actions

Proteins are probably more resistant toward proteolytic attacks in their native state and stabilizing factors (e.g., co-factor, correct parameter interval, co-solvent) are always considered optimized. Use of protein inhibitors is not recommended for safety reasons. The primary mechanism of proteolysis is the enzymatic hydrolysis of the peptide bond. The indicators of this reaction taking place in the system include loss of product or poor yield, lack of expected activity, changes in specific activity, change in MW, high background staining in ID SDS electrophoresis, smeared bands, and many lower MW bands of poor resolution, disappearance of bands, and discrepancies in MW. The preventive actions taken to prevent proteolysis are listed in Table 5. 

A topical enzyme aids in the removal of dead soft tissues by hastening the reduction of proteins into simpler substances. This is called proteolysis or a proteolytic action. The components of certain types of wounds, namely necrotic (dead) tissues and purulent exudates (pus-containing fluid), prevent proper wound healing. Removal of this type of debris by application of a topical enzyme aids in healing. Examples of conditions that may respond to application of a topical enzyme include second- and third-degree bums, pressure ulcers, and ulcers caused by peripheral vascular disease An example of a topical enzyme is collagenase (Santyl). 

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