Preparative chromatography annular

Rotating annular column An implementation of preparative chromatography using a cylindrical, annular column rotating aroimd its axis. A continuous feed stream is injected at a fixed position just above the packing. Each component band follows a helical trajectory and is collected as a continuous stream along a fixed sector of the outlet annulus. 

Finke B, Stahl B, Pritschet M, Facius D, Wolfgang J, Boehm G. Preparative continuous annular chromatography (P-CAC) enables the large-scale fractionation of fructans. J Agric Food Chem 2002 50 4743 748. 

Buchacher et al. [43] discussed the continuous separation of protein polymers from monomers by continuous annular size exclusion chromatography. The P-CAC used for the experiments was a laboratory P-CAC type 3 as described in Table 1. The results were compared to conventional batch column chromatography in regard to resolution, recovery, fouling, and productivity. The protein used in the studies was an IgG preparation rich in aggregates. Under the conditions used, the polymers could be separated from the monomers, although no baseline separation could be achieved in either the continuous or the batch mode. The... 

Figure 11.5. Schematic diagram of continuous compression columns for high-pressure Preparative-scale liquid chromatography. The arrows represent the force directions. A = radial compression, B = dynamic axial compression, and C = annular expansion.

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