Preparation of Sample Solution

Preparation of sample solution One centimeter hair sample was rinsed with 1 mL of ethanol at 37 °C for 30 min. After removing ethanol using a capillary pipette, the sample was dissolved in 1 mL of 600 mmol/L sodium hydroxide at 95 C for 10 min. Measurement of glycated proteins... 

Preparation of sample solution for detection of H2O2 in plants extract... 

Ultrapure water (18.2 MS2 cm), was obtained from a MiUipore water purification system and was used for preparation of sample solutions. Analytical Grade Acetonitrile (MeCN) (Fischer Scientific) and Trifluoroacetic Acid (TEA) (Sigma-Aldrich) were used for all ZipTip buffers (see Sect. Denaturing MS— ZipTip Buffers ). The coenzyme A derivatives (acetyl-, butyryl- and malonyl) used in assays were purchased from Sigma-Aldrich. iV-acetylcysteamine (SNAC) thioesters were provided by the Piel lab (ETH, Zurich), and stock solutions were solubilised in dimethyl sulfoxide (DMSO) (Fischer Scientific). lodoacetamide (Sigma-Aldrich) was used for all alkylation reactions. 

Preparation of sample solution To an amount of powdered sample equivalent to about 01 g of mixed sulphonamides add 50 ml of N hydrochloric acid and shake intermittently during ten minutes. Filter if necessary and dilute the filtrate and washings to 100 ml with water in a graduated flask. Transfer a 5-ml aliquot to a second 100-ml graduated flask, add 7 5 ml of N hydrochloric acid and dilute to 100 ml with water. (Solution A.)... 

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