Preparation of Protein Particles via MS Sphere Templating

Recently, a general method to prepare protein-based particles with tailored properties through MS sphere templating was reported [98]. In this approach, polyelectrolytes are infiltrated into the mesopores of MS spheres pre-loaded with 

COOH groups of the PE (PAA or PGA) and the -NH2 moieties of the pre-loaded protein, using l-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as a catalyst [99]. Negligible enzyme desorption ( 0.1 %) is observed from cross-linked lysozyme-loaded MS spheres after exposing the samples to an aqueous solution for 48 h, while about 25 % of the immobilized lysozyme is desorbed under the same conditions when the lysozyme is not cross-linked. 

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