Preparation of immunosorbents with small antigens or haptens

Preparation of immunosorbents with small antigens or haptens. It is generally advantageous to link haptens or other 

Immunoafjinity chromatography with N-hydroxysuccini-mide (NHS)-derivatized agarose. NHS ester-derivatized cross- 

The gel to be coupled is suspended uniformly, transferred to a sintered glass funnel and washed with 3 volumes of iso-propanol and 3 volumes of cold distilled water (less than 20 min). The cold ligand solution (0.5-1 volume) in 100 mM NaHCOsis mixed gently with the gel for 1 h at room temperature. This is followed by an incubation (1 h) with ethanolamine-HCl buffer, pH 8.0. The gel is washed and ready for use. Ligands not soluble in water may be used in methanol, ethanol, acetone or DMF. 

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