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Preparation of Fab fragments

In some situations. Fab fragments (Section 14.4.2) are preferred over the complete Ig or are even essential for EIA. [Pg.117]

Preparation of Fab Fragments from IgGs of Different Animal Species... [Pg.142]

Rousseaux, J., Rousseaux-Prevost, R., and Bazin, H. (1983) Optimal conditions for the preparation of Fab and F(ab )2 fragments from monoclonal IgG of different rat JgC subclasses. J. Immunol. Meth. 64, 141-146. [Pg.1109]

One limitation to this method should be noted. If the antibody-enzyme conjugate is prepared using antibody fragments such as Fab or F(ab )2, then nickel-chelate affinity chromatography will not work, since the requisite Fc portion of the antibody necessary for complexing with the metal is not present. [Pg.815]

Coulter, A., and Harris, R. (1983) Simplified preparation of rabbit Fab fragments./. Immunol. Meth. 59,... [Pg.1056]

Ghetie, V., Ghetie, M.-A., Uhr, J.W., and Vitetta, E.S. (1988) Large scale preparation of immunotoxins constructed with the Fab fragment of IgGl murine monoclonal antibodies and chemically deglyco-sylated ricin A chain./. Immunol. Meth. 112, 267-277. [Pg.1066]

Masuho, Y., Hara, T., and Noguchi, T. (1979) Preparation of hybrid of fragment Fab of antibody and fragment A of diphtheria toxin and its cytotoxicity. Biochem. Biophys. Res. Comm. 90, 320. [Pg.1092]

Motta-Hennessy, C., Eccles, S.A., Dean, C., and Coghlan, G. (1985) Preparation of 67Ga-labeled human IgG and its Fab fragments using deferoxamine as chelating agent. Eur. J. Nucl. Med. 11, 240-245. [Pg.1096]

See other pages where Preparation of Fab fragments is mentioned: [Pg.808]    [Pg.61]    [Pg.500]    [Pg.54]    [Pg.143]    [Pg.145]    [Pg.147]    [Pg.149]    [Pg.524]    [Pg.95]    [Pg.117]    [Pg.480]    [Pg.103]    [Pg.808]    [Pg.61]    [Pg.500]    [Pg.54]    [Pg.143]    [Pg.145]    [Pg.147]    [Pg.149]    [Pg.524]    [Pg.95]    [Pg.117]    [Pg.480]    [Pg.103]    [Pg.223]    [Pg.17]    [Pg.10]    [Pg.111]    [Pg.100]    [Pg.299]    [Pg.13]    [Pg.517]    [Pg.3]    [Pg.68]    [Pg.252]    [Pg.445]    [Pg.483]    [Pg.131]    [Pg.284]    [Pg.292]    [Pg.786]    [Pg.808]    [Pg.826]   


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