Preparation of Enterobacter sp. Resting Cells with High Degradation Activity

1 Cultivation and Preparation of Enterobacter sp. Resting Cells with High Degradation Activity 

The method of how cells with high activity are cultured and obtained is a most important and most critical subject. For preparing cells of Enterobacter sp. DS-S- 

the following factors were investigated 1) medium composition and concentration, 2) growth temperature, and 3) concentration of oxygen. 

First of all, we generally used a nutrient medium composed of peptone, yeast extracts, and glycerol when culturing several of the bacteria we had isolated. The yeast extract, namely its type and lot, was found to be a major factor so we needed to select the most stable variety (Fig. 14). 

Secondly, it often occurred that the optimal temperature for cell growth and the temperature for highest activity were different. Because the total activity of the resting cells can be shown to be a product of cell amounts and activity per unit volume, both high specific activity and high concentration of the cells were required when culturing the resting cells (Fig. 15). 

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