Preparation of Agar Media

Choose one formula, mix the ingredients in dry form, place into a flask and add water until one liter of medium is made. 

PDY (Potato Dextrose Yeast) Agar the filtered, extracted broth from boiling 300 grams of sliced potatoes in 1 liter of water for 1 hour20gramsagar 

MEA Malt Extract Agar) 20 grams tan malt 2 grams yeast 

20 grams tan malt 5 grams ground rye grain 5 grams peptone or neopeptone 2 grams yeast (optional) 

For controlling bacteria, 0.10 grams of 60-80% pure gentamycinsulphatecan be added to each liter of media prior to sterilization. (See Resources in Appendix.) 

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