Preparation and Extraction of Tissues

Samples are extracted prior to injection onto the column. This serves two major purposes (a) solid tissue components are removed to prevent 

Wash AI2O3 with H2O + Tris buffer (10 ml/liter) 

Desorb catecholamines into 0.1 M or 60 mM HCIO4 by gentle agitation (5 min) 

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