Predictions for Proteins with Known 3D Structure

Insufficiently Reliable SWISS-PROT Structures of Membrane Proteins 

In some cases, the prediction that can now be obtained by means of the pref method (2.0 version) is sufficient to correct the error in the number and location of transmembrane segments and consequently the error in the topology of the membrane protein. However, for some other proteins it is necessary to use the positive inside rule, similarity with related proteins as well as predictions obtained by other methods. 

Predictions for Proteins for Which, According to the SWISS-PROT Data Bank, Structure and Topology Are Not Known 

The profile contains nine very clear transmembrane segments with very high maxima which also correspond by length fully to transmembrane segments. The distribution of positively charged amino acid side chains in the extramembrane part 

---