Precipitin reaction, quantitative studies

By the quantitative study of precipitin reactions Heidelberger and Kendall" reached the same conclusion, and showed in addition that even after prolonged immunization the antiserum studied (anti-egg albumin) contained much low-grade antibody, incapable by itself of forming a precipitate with the antigen, but with the property of being carried down in the precipitate formed with a more reactive fraction."... 

Somewhat similar quantitative theories of the precipitin reaction have been published by A4. Heidelberger and F. B. Kendall, J. Bxfitl. Mfd., 61, 5G3, 62, 467, 697 (1935) 66, 229 (1937) P. E. Kendall, Annals N, V. Acad. Sc4., 163,85 (.1942 and A. D. Hershey, J. Immunol., 42, 465 (1941). These theories are designed to apply more broadly than ours, which is based on postulates suited to the special antigens and haptens which we are studying. 

L.C. Wetter, M.Cohn and H.F.Deutsch, Immunological Studies of Egg-white Proteins. II. Resolution of the Quantitative Precipitin Reaction between Chicken Egg-white and Rabbit Anti-egg-white Serum in Terms of the Reactions of Purified Egg-white Proteins, J. Immun. 69, 109-115 (1952). 

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