Porcine liver molecular weight

Figure 2. SDS gel electrophoresis of the products of partial cystine cleavage for several test proteins. A. molecular weight standards, B. yeast alcohol dehydrogenase. C. P-lactoglobulin, D. hen egg lysozyme, E. ovalbumin, F. calf fetal serum fetuin. Molecular weight standards are indicated by arrows on the left side of the gel and are bovine serum albumin (66,300), bovine liver glutamate dehydrogenase (55,400), porcine muscle lactate ddiydiogenase (36,500), bovine erythrocyte carbonic anhydrase (31,000), soybean trypsin inhibitor (21,500), hen egg lysozyme (14,400), bovine lung aprotinin (6,000), unresolved bovine pancreatic insulin A and B chains.

Later on, FAAH was cloned from human and mouse liver (Giang and Cravatt, 1997), and from porcine brain (Goparaju et al., 1999b). All these enzymes are composed of 579 amino acids, and their molecular weights are 63 kDa. The porcine enzyme shows 80, 81, and 85% identity of the deduced amino acid sequence to mouse, rat and human FAAH respectively (Goparaju et al., 1999b). 

Affinity chromatography of human liver hydrolases Fractionation of low molecular weight porcine heparin into active and relatively inactive forms Affinity chromatography of heparin Affinity chromatography of rabbit anti-thyroglobulin antibodies Purification of Phaseolus vulgaris isolectins Purification of in vivo activity of an H-Z allo-antiserum... 

Fig. 9.1. Linear relationship between molecular weight logarithm of globular proteins and log n (number of antigenic determinants) (from Louvard et ai, 1976). Abbreviations are as follows RNase pancreatic ribonuclease OV, ovalbumin BSA, bovine serum albumin yG, human IgG immunoglobulin PH, E. coli alkalin phosphatase Cat, liver catalase AP, porcine intestinal aminopeptidase. The following equations apply s = k s — /c2U, log M =...

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