Polyspher

HPLC analysis of furosine (-peak II) in hydrolyzates of non-exposed- (bottom), buffer-exposed (middle), and glucose-exposed (top) dentin samples. Dentin was not reduced prior to hydrolysis. Only the relevant parts of the chromatograms are shown. Amino acids are visualized after post-column labelling with a fluorescent dye. I lysine, II furosine. III homoarginine (internal standard). Column Merck Polyspher AA-NA 120 x 4.6 mm flow 0.2 ml/min gradient pH 5.0 -10.2 postcolumn reagent 0.2 ml/min fluorescence Xgx 330 nm, 440 nm 100-yl injections in buffer pH 2. 

The HPLC gradient system consisted of two pumps for solvent delivery, an injector, a 120 x 4.6 mm Polyspher AA-NA column (Merck, 75°C), a pump for reagenf delivery, a mixing tee with reaction coil, and a fluorescence detector lined to an integrator (Waters). 

Parameter Metrosep anion dual 2 TSK-Gel IC-PW Shimpack IC-Al Polyspher ICAN-1 Universal anion... 

Polyspher IC AN-1 from Merck AG (Darmstadt, Germany), consisting of a hydrophilic polymethacrylate packing with a particle diameter of 12 pm, exhibits a similar selectivity. Depending on the type of application, this column can be operated with eluents based on phthalic acid, / -hydroxybenzoic acid, tartaric acid, oxalic acid, or boric acid. Figure 3.19 shows a typical chromatogram obtained with a phthalic acid eluent. Under these chromatographic conditions (pH 4), orthophosphate elutes as a monovalent anion ahead of chloride. 

Figure 3.19 Separation of various inorganic anions on Polyspher 1C AN-1. Column temperature 35 °C eluent 1.5mmol/L phthalic acid -h 1.38 mol/L Tris -h 0.3 mol/L H3BO3, pH 4.0 flow rate 1.3 mL/min ...

Separator TSK-Gel > LCA A03 Shimpack Polyspher Super-Sep Metrosep Allsep Anion Allsep A-2 MCI SCA04... 

Separator TSKMetrosep Anion Dual 1 Metrosep Anion Dual 2 Polyspher ICAN-1 Univeisal Anion MCISCA04... 

Fig. 9-25. Separation of aliphatic carboxylic acids in the seepage water of a sewage sludge dump. — Separator columns (a) Polyspher OA-HY (Merck), (b) lonPac ICE-AS6 (Dionex) column temperatures (a) 45°C, (b) 60°C eluants (a) 5 mmol/L H2SO4, (b) 0.4 mmol/L perfluorobutyric acid flow rates (a) 0.5 mL/ min, (b) 0.5 mL/min detection (a) UV (219 nm), (b) suppressed conductivity injection volumes (a) 20 pL, (b) 25 pL sample preparation OnGuard-P peaks (a) (1) strong acids, (2) pyruvic acid, (3) glyoxylic...

Separator column RT 300-6.5 Polyspher CHCA column temperature 85 °C eluant deionized water flow rate 0.6 mL/ min detection Rl injection volume 20 pL sample dry white wine with a total sugar content of 0.1 g/L, spiked with tartaric acid, lactic acid, and malic acid to yield 17.6 g/L total acid peaks ... 

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