Polysaccharides agarose

N.A. Gelidium cartilagineum L. Polysaccharides, agarose, agaropectin, mucilage.99 Laxative, stimulating bowel activity and elimination of feces. 

Mucilaginous substance extracted from red marine algae (Rhodophyceae). Used as protective colloid in photometric detn. of Sn, Mg, Si, S04 . Used as a solid medium for the cultivation of bacteria. A thickener, stabiliser and gelling agent in the food industry. Strips or fine powder. SI. sol. in hot H2O. Consists of a mixt. of 2 polysaccharides, agarose and agaropectin. 

Purifications are made simpler with Toyopearl HW media because there is no risk of leached polysaccharides to contaminating eluted fractions. Saccharide derivatives have been known to leach from conventional low-pressure column packings, such as dextran or agarose gels. 

Agarose gels have been used for more than two decades to separate polysaccharides (17-22). In particular, Sepharose CL 2B is widely used (6-8) to separate native starch, but continuously improved mechanical and chemical stability made all of the Sepharose CL gels perfect systems for the analysis of high molecular and broad distributed polysaccharides (23-28). 

Agar is made up of two polysaccharides, agaropectin and agarose. As with carrageenan, the backbone of these polysaccharides is the sugar galactose. 

In the previously described electrophoretic methods, the capillary was filled with electrolytes only. Another mode of operation in capillary electrophoresis involves filling the capillary with gel or viscous polymer solutions. If desired, a column can be packed with particles and equipped with a frit.68 This mode of analysis has been favorably used for the size determination of biologically important polymers, such as DNA, proteins, and polysaccharides. The most frequently used polymers in capillary gel electrophoresis are cross-linked or linear polyacrylamide,69 cellulose derivatives,70-75 agarose,76 78 and polyethylene glycols. 

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