Polypeptides Raman optical activity

III. Survey of Polypeptide and Protein Raman Optical Activity. 59... 

Raman optical activity is an excellent technique for studying polypeptide and protein structure in aqueous solution since, as mentioned above, their ROA spectra are often dominated by bands originating in the peptide backbone that directly reflect the solution conformation. Furthermore, the special sensitivity of ROA to dynamic aspects of structure makes it a new source of information on order-disorder transitions. 

Vitalis and Pappu have applied experimental /hohp couplings for dipeptides as one of the tests to assess the validity of the ABSINTH model, their new continuum solvation model for simulations of polypeptide structures in aqueous solutions. Theoretical and experimental vicinal proton-proton couplings have been found by Bouo and co-workers to be quantitatively consistent with the Raman optical activity of four proline containing model dipeptides. 

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