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Polynucleotides and Single-Stranded DNA Fragments

A closer examination of the Okazaki fragments led to detection of short stretches of ribonucleotides at the 5 ends. From this and many other observations on different systems it was determined that a new DNA chain can be initiated only by attaching the first deoxynucleotide through its 5 -phosphate to the 3 -OH of a short RNA polynucleotide. An RNA strand that functions in this capacity is called a primer. Primers are formed at points along the chromosome they base-pair with the single-stranded template DNA in the regions where they are formed. [Pg.653]

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DNA fragmentation

DNA fragments

DNA strand

Polynucleotide

Polynucleotides

Polynucleotides, single-stranded

Single-strand

Single-stranded

Single-stranded DNA

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