Polymethylmethacrylate beads

Mader J T, Calhoun J, Cobos J 1997 In vitro evaluation of antibiotic diffusion from antibiotic-impregnated biodegradable beads and polymethylmethacrylate beads. Antimicrobial Agents and Chemotherapy 41 415-418... 

Cooper, E.C. and Vincent, B. (1989) Encapsulation of filler particles in polymethylmethacrylate beads by a doubledispersion method. Journal of Colloid and Interface Science, 132,592-594. 

K. Adams, L. Couch, G. Cierny, J. Calhoun and J.T Mader, In vitro and in vivo evaluation of antibiotic diffusion from antibiotic-impregnated polymethylmethacrylate beads. Clin. Orthop. Relat. Res. 278 244-252,1992. 

Another unique silica-based approach to microscale DNA extraction currently underdevelopment utilizes a serpentine channel design, combined with an immobilized silica-bead solid phase and fluidic oscillation. This method, developed by Chung et al., relies on silica beads immobilized on the plasma-oxidized surface of the polymethylmethacrylate (PMMA) channels, instead of a packed-silica solid phase, as depicted in Figure 43.1c. Following bead immobilization, the solutions required for DNA binding, purification, and release are flowed back-and-forth through the device. This fluidic oscillation over the immobilized phase results in marked improvement of recovery and extraction efficiency over the same extraction methods with free beads. This method represents yet another variation of silica-based purifications that has been accomplished in microfluidic systems, exploiting previously optimized chemistries. In summary, the development of macroscale, commercial, silica SPE protocols has enabled the facile translation of DNA, and now RNA, extraction into microfluidic systems for a variety of applications. 

Embedded debris. Hood and coworkers initially restricted this damage mode to polymethylmethacrylate (PMMA) debris, but it is possible that bone chips or metallic beads or fragments from the back surface of metallic components could... 

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