Polymer-oligodeoxynucleotides

In protozoa this problem is solved by the addition of preexisting oligodeoxynucleotide blocks to the 3 ends of DNA. These blocks are composed of tandemly repeated units of (T2G4)n or (T4G4)n, where n is approximately 50. The enzyme that adds these polymers requires a primer but not a template. These oligonucleotide block polymers are called telomers. Another DNA polymerase,... 

Katzhendler J, Cohen S, Weisz M, Ringel I, Camerini-Oetrio RD, Deutsch A. Spacer effect on the synthesis of oligodeoxynucleotides by the phosphite method. R ct Polym 6 175-187, 1987. 

Koster H, Stumpe A, Wolter A. Polymer support oligonucleotide synthesis Rapid and efficient synthesis of oligodeoxynucleotides on porous glass support using the triester approach. Tetrahedron Lett 24 747-750, 1983. 

The time required for MSNT-mediated couplings can be considerably shortened by the addition of 7V-methylimidazole (NMI). For example, the time for the monomer (as the triethylammo-nium salt of the phosphodiester) addition in a solid-phase synthesis of oligodeoxynucleotides performed on controlled pore glass can be reduced to less than 10 min when MSNT is used in combination with NMI. A large-scale soluble polymer... 

The requirement for the new approaches to control the delivery of the compounds such as peptides, proteins, plasmid DNA, antisense oligodeoxynucleotides, and immunotoxins has been created through the development of different advanced medications over the previous decade. Their ability to get the targeted regions is important for the activity of these molecules, nevertheless, once they enter into the body system proteases or DNA-degrading enzymes in vivo simply degraded the mentioned polymers [65]. 

Synthesis of Oligodeoxynucleotides on Polymer Supports Using the Diester Approach"... 

Koster, H., Biernat, J, McManus, J., Wolter, A, Stumpe, A. Narang, C. K, and Sinha, N D (1984) Polymer support oligonucleotide synthesis, 15, synthesis of oligodeoxynucleotides on controlled pore glass (CPG) using phosphate and a new phosphite triester approach Tetrahedron 40, 103-112... 

---