Polyhydroxybutyrate biosynthesis pathway

Escherichia coli Succinate and polyhydroxybutyr-ate (PHB) A/B Since PHA/PHB is an intracellular compound and succinate is an extracellular compound, they can be easily separated, either by centrifugation or filtration. The NADPH consumed during PHB biosynthesis can be regenerated by pushing the metabolic pathway toward the TCA cycle. 32,33... 

John, M. E. and Keller, G. (1996). Metabolic pathway engineering in cotton Biosynthesis of polyhydroxybutyrate in fiber cells. Proceeding of National Academy of Science. USA 93, 12768-12773. 

Jendrossek D, Backhaus M, Andermann M (1995) Characterization of the Comamonas sp. poly(3-hydroxybutyrate) (PHB) depolymerase and of its structural gene. Can J Microbiol 41 160-169 Jendrossek D, Schirmer A, Schlegel HG (1996) Biodegradation of polyhydroxyalkanoic adds. Appl Microbiol Biotechnol 46 451—463 John ME, Keller G (1996) Metabolic pathway engineering in cotton biosynthesis of polyhydroxybutyrate in fiber cells. Proc Natl Acad Sd USA 93 12768-12773... 

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