Polyethylene oxide electrophoresis

Guttman, A., Shieh, P, Lindahl, J., and Cooke, N., Capillary sodium dodecyl sulfate gel electrophoresis of proteins. II. On the Ferguson method of polyethylene oxide gels, /. Chromatogr., 676, 227, 1994. 

Three bacterial species (E. coli, P. putida, and S. rubidae) were separated on isoelectric focusing in methylcellulose coated capillaries, and three bacterial species (P. fluorescens, E. aerogenes, and M. luteus) and the yeast S. cerevisae, were separated by capillary electrophoresis in the presence of polyethylene oxide.101 The polymers served to minimize adsorption to the walls without causing cellular lysis. 

Figure 9.8 Ferguson plots for polyethylene oxide used as sieving polymer. The graphs represent plots of the polymer concentration and the log of electrophoretic mobility for six different samples. Graphs A, B, and C represent polymers with MW of 100, 300, and 900 kDa, respectively (From A. Guttman, Electrophoresis, 16 611 (1995). With permission.)...

Polyethylene oxide (PEO) and polyvinylpyrrolidone (PVP) solutions both proved to be good separation matrices in capillary electrophoresis-based DNA sequenc-... 

Complex carbohydrates released from glycoproteins were readily profiled by capillary gel electrophoresis with LIF-based detection of 1-aminopyrene-3,6,8-trisulfonic acid (APTS)-labeled sugar molecules (Figure 10) [125]. High-mannose-type oligosaccharides of ribonuclease B were derivatized by APTS and separated by capillary electrophoresis using polyethylene oxide separation medium [126]. 

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