Polyethylene glycols capillary electrophoresis

In the previously described electrophoretic methods, the capillary was filled with electrolytes only. Another mode of operation in capillary electrophoresis involves filling the capillary with gel or viscous polymer solutions. If desired, a column can be packed with particles and equipped with a frit.68 This mode of analysis has been favorably used for the size determination of biologically important polymers, such as DNA, proteins, and polysaccharides. The most frequently used polymers in capillary gel electrophoresis are cross-linked or linear polyacrylamide,69 cellulose derivatives,70-75 agarose,76 78 and polyethylene glycols. 

Cunico R L, Gruhn V, Kresin L, et al. (1991). Characterization of polyethylene glycol modified proteins using charge-reversed capillary electrophoresis. /. Chromatog. 559 467-477. 

Li W, Zhong Y, Lin B, Su Z (2001). Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis. J. Chromatog. A. 905 299-307. 

Figure 2 Serum electrophoresis of a patient with a small monoclonal band (top) CE (bottom) agarose electrophoresis l = internal standard A = albumin ai =ai-globulin a2 = a2-glob-ulin, T = transferrin C = complement M = monoclonal band > = >-globulins X = marker). CE conditions capillary 30cmx 50 pm (ID), 9kV, detection at214nm. Separation buffer 7g boric acid, 7g sodium carbonate, and 5 g of polyethylene glycol 8000 in 1000 ml water.

Fig. 5 Extraction of analytes with organic solvents (A) Benzoic acid standard 30 mg/L in the electrophoresis buffer (separation under non-stacking) and (B) Benzoic acid 30 mg/L in 10 mmol/L HCl extracted with chloroform (containing 10% methanol) with direct chloroform injection for 40 sec on a capillary 50 jjim x 40 cm, 214 nm, and 12 kV. Separation buffer boric acid 400 mg, sodium carbonate 200 mg, polyethylene glycol 200 mg in 100 ml water. (B = benzoic acid.)...

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