Polyene macrolides, analysis

Configurational assignment of polyene macrolide antibiotics using the [ C]ace-tonide analysis 98ACR9. 

The 13C NMR spectrum of 64, an amide of 63, showed sixty-two carbon signals of which partial assignments, shown in Table 16, were made based upon distortionless enhancement by polarization transfer(DEPT), H-13C correlation experiments and literature data describing 13C NMR analysis of polyene macrolides. 

In recent years, much effort has been spent trying to determine the absolute configuration of the many polyene macrolide asymmetric centers, using well-established enantioselective organic transformations and advanced spectroscopic studies. This work is a necessary prelude to any accurate chemical modification, conformational analysis related to biological activity and, of course, attempt to totally synthesize a given antibiotic or analogue. 

Rychnovsky, S. D., B. N. Rogers, and T. I. Richardson, Configurational Assignment of Polyene Macrolide Antibiotics Using the [ C] acetonide Analysis, Accounts of Chemical Research 31 (1998) 9-17. 

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