Polyacrylic resins with immobilized enzyme

The immobilized thermolysin was prepared by adsorption of the enzyme onto the supports, polyacrylic ester resins (AmberUte series) and pore glass particles (CPG series), followed by crosslinking with glutaraldehyde in aqueous solutions. The activity of the immobilized enzyme was assayed by measuring the initial synthetic rate of fV-(benzyloxycarbonyl)-L-phenylalanyl-L-phynylalanine methyl ester in the biphasic reaction system. 

Enzymatic polymerization has emerged in the last few decades as a field of considerable interest and commercial promises. It proceeds with high regio-, enantio-, and chemos-electivity under relatively mild conditions. So far, enzymes have been used to synthesize polyesters, polysaccharides, polycarbonates, polyphenols, polyanilines, vinyl polymers, and poly(amino acid)s. Namely, the lipase B of Candida antarctica (Cal-B, a serine hydrolase) immobilized on polyacrylic resin (Novozyme 435) has proven to be a very versatile catalyst in terms of reaction conditions and acceptance of various substrates. For example, this enzyme has been successfully used to synthesize polyesters. ° However, little has been reported so far on the synthesis of polyamides catalyzed by enzymes. " ... 

---