Polyacrylamide-silastic tubing

Figure 7a. ESC A C Is spectrum of the luminal surface of polyacrylamide-Silastic tubing in the dehydrated state.

Table II shows the effect on platelet consumption of increasing lengths of the polyacrylamide-Silastic tubing. The acrylamide surface is much more destructive to platelets than is the Silastic surface. Table II also shows that platelet consumption increases linearly with shunt area for both the polyacrylamide-Silastic shunts.

Protein Adsorption. Since protein adsorption is the earliest event in the thrombotic response of the blood to polymers, the types of proteins adsorbed from plasma to polyacrylamide-Silastic and Silastic tubes were examined. As described in the Experimental section, plasma was recirculated through the tubes for 2 h at 37°C and rinsed away with buffer. The proteins adsorbed to the polymers were eluted with SDS, separated by SDS-polyacrylamide slab gel electrophoresis, and silver-stained using a new extremely sensitive method (19). Figure 10 shows the results of these assays. 

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