Polyacrylamide containing nucleic acid

Polyacrylamide and Polymethacrylamide Containing Nucleic Acid Bases... 

The initial characterizations of the various fusion proteins (Subheading 3.1.1, step 7), as well as more detailed biochemical assays (Subheading 3.3) make extensive use of electrophoretic separations and immunoblotting. Protocols used in the authors laboratories for these various techniques (SDS-polyacrylamide gel electrophoresis, native acrylamide gel electrophoresis, and separation of nucleic acid on acrylamide gels containing urea) are described in this section. 

In 1970, Weith and co-workers first prepared boronic acid-functionalized chromatographic media via immobilizing APBA to cellulose for the separation of nucleic acid components and carbohydrates. Since then, various boronate affinity chromatographic media such as cellulose, sephacryl, sepharose, polyacrylamide and silica beads have been developed because of the merits of boronate affinity for capturing cis-diol-containing biomolecules. Various matrices and boronic acid derivatives are listed in Table 11.3. It can... 

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