Poly synthetase molecular weight

Table 1 is a summary of the amino acid composition of poly(ADP-ribose) synthetase. It reveals that the enzyme consists of 1,014 amino acid residues with a calculated molecular weight of 113,153. The total amino acid composition calculated from the deduced amino acid sequence coincided well with that determined experimentally. 

Fig. 1. Estimation of the size of mRNA for bovine poly(ADP-ribose) synthetase by in vitro translation of size fractionated poly (A) + RNA and Northern blot analysis. A. Size-fractionation of poly (A) + RNA and location of mRNA for the enzyme. Bovine thymus poly(A)+ RNA was size-fractionated by neutral sucrose density gradient centrifugation and RNA in each fraction was translated in vitro. The translated products were immunoprecipitated, and separated on a 7.5% SDS-polyacrylamide gel. B. Typical fluorogram of the gel. Lanes 1, 2, 3, 4, and 5, correspond to fractions 1, 3, 5, 7, and 9, respectively. Molecular weight markers a, p-galactosidase (116K), b, phosphorylase a(95K), c, bovine serum albumin (68K), d, ovalbumin (43K), e, lysozyme (14.3K). C. Northern blot analysis of bovine thymus poly(A)+ RNA. RNA (2 pg per lane) was separated on a 1.2% agarose/formaldehyde gel, transferred to a nitrocellulose filter, and hybridized with the p-iabelled 2.7 kb insert cDNA prepared from the clone ARS-1. Size markers used were 28 S(4.9 kb), 18 S(2.0 kb)rRNA, 3.8 kb, 2.7 kb, and 1.1 kb denatured DNA. Reprinted from Taniguchi etaL, Eur J Biochem 171 571-575,1988.

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