PNGase

Glycoprotein was digested using the peptide N-Glycosidase F (PNGase F) to release N-linked glycan, then labeled with 8-aminopyrene-l,3,6-trisulfonate (APTS). The detection used LIF with a argon-ion laser. 

Figure 6.7 Comparative peptide map of enzymatic digests of bovine ribonuclease B. (A) TIC trace of digested RCM-glycoprotein with trypsin. (B) TIC trace of digested RCM-glycoprotein with trypsin and PNGase F. (Reprinted with permission from Liu et al., 1993. Copyright 1993 Elsevier.)...

Identification of the 5-HT2C receptor as a 60-kDa N-glycosylated protein in the choroid plexus and hippocampus has also been carried out in another study (145). When rat choroid plexus and hippocampal membrane lysates were treated with PNGase F, 5-HT2C receptor polypeptides from both tissues were converted to a sharp 38-kDa band. In a time-course experiment performed in the choroid plexus,... 

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