Plunger measurement procedure

It is possible, however, to measure a so-called response factor for the analyte, which is the area generated by a unit quantity inj ected, such as a micro liter or microgram. The procedure is to inject a known quantity of the analyte, measured by the position of the plunger in the syringe (microliters) or by weighing the syringe before and after injection (micrograms). The peak size that results is measured and divided by this quantity ... 

In the present study, three groups of six cattle (a total of eighteen) were administered the Captec device intra-ruminally via a fistula. Drug release rates were determined by HPLC assay of ABZ and its major metabolites in plasma as well as by physical measurement of the plunger movement. ABZ marker residue levels were determined using the established regulatory procedure in total liver, an ethyl acetate extractable fraction as well as the remaining intractable (bound) residue on each of three cattle at 0- and 5-Day withdrawal. 

A 3-minute fixed-time assay will be used to measure peroxidase activity. Two tubes will be set up for each assay, one tube for the zero point and the other tube for the 3-mmute assay. The amount of gel-immobihzed peroxidase will be different for each assay. Obtain six test tubes for reaction vessels and label them 1-6. Weigh two samples of 0.05 g of immobilized peroxidase and transfer one sample to tube 1 and the other sample to tube 2. To tubes 1 and 2, add 2 5 mL of the aminoantipynne-phenol solution. Mix well. To tube 1 (zero point), add 2.5 mL of the solution, rapidly mix well, and immediately quench the reaction by pouring into the barrel of a syringe with filter system. Use the plunger to force the solution through the filter into a glass cuvette. This whole procedure from mixing to transfer should take no more than 10 seconds. Read and record in your notebook the absorbance of the reaction mixture at 510 nm. 

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