Platelet storage

Huang L, Kuo YM, Gitschier J (1999) The pallid gene encodes a novel, syntaxin 13-interacting protein involved in platelet storage pool deficiency. Nat Genet 23 329-332... 

Novak EK, Hui S-W, Swank RT. 1984. Platelet storage pool deficiency in mouse pigment mutations associated with seven distinct genetic loci. Blood 63 536-544. 

Reddington M, Novak EK, Hurley E, Medda C, McGarry MP, et al. 1987. Immature dense granules in platelets from mice with platelet storage pool disease. Blood 69 1300-1306. 

Swank RT, Reddington M, Novak EK. 1991a. Platelet storage pool deficiency associated with inherited abnormalities of the inner ear in the mouse pigment mutants muted and mocha. Blood 78 2036-2044. 

Swank RT, Sweet HO, Davisson MT, Reddington M, Novak EK. 1991b. Sandy A new mouse model for platelet storage pool deficiency. Genet Res 58 51-62. 

Secreted constituents of platelet storage granules, particularly ADP, amplify primary aggregation stimulated by weak agonists. An important event necessary for TP receptor-... 

KOERNER TA, Cunningham MT, Zhang DS. The role of membrane lipid in the platelet storage lesion. Blood Cdh 18 481-97 discussion 498-500,1992. 

A platelet storage pool deficiency has been reported in a colony of pigs with von Willebrand s disease and in heterozygous carriers (Daniels et al. 1986). Platelet aggregation in re onse to collagen is reduced in most affected pigs. Dense bodies ate markedly reduced in number and total platelet content of ADP, ATP, and serotonin is decreased. 

DanidsTD,FassDN, White JG, Bowie EJW. Platelet storage pool defidency in pigs. Blood 1986 67 1043-1047... 

Meyers KM, Seadiord CL, Holmsen H, Prieur D J. Evaluation of the platelet storage pool deficiency in the feline counterpart of Chediak-Higashi syndrome. AmJHematol 1981 11 241-248... 

For optimal functionaUty, platelets require a stable and weU-balanced pH, gas exchange, ambient temperature, and gentle agitation. Special plastics have been developed for optimal storage of platelets. 

Benzene zero 0.005 Anemia decrease in blood platelets increased risk of cancer Discharge from factories leaching from gas storage tanks and landfills... 

Chromaffin granules, platelet dense core vesicles, and synaptic vesicles accumulate ATP. ATP uptake has been demonstrated using chromaffin granules and synaptic vesicles and the process appears to depend on A(.lh+. It has generally been assumed that ATP is costored only with monoamines and acetylcholine, as an anion to balance to cationic charge of those transmitters. However, the extent of ATP storage and release by different neuronal populations remains unknown, and the proteins responsible for ATP uptake by secretory vesicles have not been identified. 

The chemical engineering solution was to design a new material for the storage bag that was capable of "breathing"—of allowing carbon dioxide to diffuse out and oxygen to diffuse in. This prevents the drop in pH. Platelets stored in this new bag survive 10 days or more. 

Platelets adherent to collagen change shape and spread out on the subendothelium. They release the contents of their storage granules (the dense granules and the alpha granules) secretion is also stimulated by thrombin. 

Many of the coagulation factors measured by global coagulation tests have limited stability, and the time and temperature of storage of sample will affect their measurements. Concepts of analyte stability and half-life in plasma extend to markers measured by immunoassay. Markers of platelet activation are affected by artifactual activation in vitro upon collection of the blood specimen. This section will highlight some of the nonanalytical variables that, if uncontrolled, can lead to spurious results and thus affect the interpretation of laboratory data. 

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