Plasticity protein interactions

A key feature of the solid-phase ELISA is that antigens or antibodies can be attached to smfaces easily by passive adsorption. This proeess is commonly called coating. Most proteins adsorb to plastic smfaces, probably as a result of hydrophobie interactions between nonpolar protein substructures and the plastie matrix. The interactions are independent of the net charge of the protein, and thus each protein has a different binding constant. The hydrophobieity of the plastic/protein interaction can be e qjloited to increase binding since most of proteins hydrophilic residues are at the outside and most of the hydrophobic residues orientated towards the inside (1). 

Because of the noncovalent nature of the plastic/protein interactions, desorption (leaching) may take place dining the stages of the assay. However, if conditions are standardized, leaching does not affect the viability of the majority of tests. There are some reports that the vigorousness of washing at the... 

Non-living arrays Synthetic peptides on plastic Synthetic peptides on paper Affinity-purified proteins protein interactions, epitope mapping protein interactions, epitope mapping, and amino acid specificities of glycation reactions biochemical activities... 

Sundberg, E. J., and Mariuzza, R. A. (2000). Luxury accommodations The expanding role of structural plasticity in protein-protein interactions. Structure Fold Des. 8, R137-R142. 

Figure 6. Network representation of MASC proteins with phenotypic annotation 97 MASC proteins with known direct protein interactions to other MASC proteins are plotted. The NMDA receptor subunits (NRl, NR2A, NR2B) are centrally located. The association of each protein with plasticity, rodent behaviour or human psychiatric disorders is shown in the colour of the node. Key Black - no known association Red - Psychiatric Disorder Green -Plasticity Blue - Rodent Behaviour Yellow - Psychiatric Disorders and Plasticity Cyan -Plasticity and Rodent Behaviour White - all three phenotypes. Orange for Psychiatric Disorders and Rodent Behaviour alone had no hits.

There is some evidence that protein molecules in high concentrations do not unfold totally, since the protein-protein interactions overwhelm the ability of the protein to compete for water hydrogen bonds. We can say that proteins in food are water -plasticizable amorphous polymers. In fact a number of proteins are not water soluble, but they become plasticized by water in the amorphous state. 

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