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PITC

FIG. 21-27 Screw feeder, It needs a variable-pitcli screw to produce a uniform draw of material, Couitesif of Chemical Engineering,)... [Pg.1940]

Nucleophilic addition of the peptide terminai amino group to p nenyi isothiocyanate (PITC) gives an /V-phenylthiourea derivative. [Pg.1032]

PITC (Section 26.6) Phenylisothiocyanate used in the Edman degradation. [Pg.1247]

HT29 (colorectal) CHO (Chinese hamster ovary) SVM(muntjac cells) BITC > PEITC PEITC > AITC AITC > BITC > PEITC > PITC Musk et al. (5)... [Pg.56]

HeLa PHITC > PEITC = PBITC = PMITC > PITC Yu et al. (81)... [Pg.56]

Abbreviations DEPC, diethylpyrocarbonate DCCD, Af.iV -dicyclohexylcarbodiimide EEDQ, N-ethyoxycarbonyl-2-ethoxy-1,2-dihydroquinoline NEM, A-ethylmaleimide PAO, phenylarsine oxide NPM, IV-phenylmaleimide PLP, pyridoxal phosphate DIDS, diisothiocyanostilbene disulfonate PITC, phenylisothiocyanate. [Pg.250]

Two other reagents used in HPLC are 9-fluorenyl methoxycarbonyl chloride (FMOC) and phenylisothiocyanate (PITC). 9-fluorenyl methoxycarbonyl chloride is becoming increasingly popular in protein chemistry research because it reacts with secondary amines and also offers rapid analysis of protein hydrolysates. [Pg.50]

PITC has been used extensively in the sequencing of peptides and proteins and reactions under alkaline conditions with both primary and secondary amino acids. The methods of sample preparation and derivatization follow a stringent procedure which involves many labour-intensive stages. However, the resulting phenylthio-carbamyl-amino acids (PTC-AA s) are very stable, and the timing of the derivatization step is not as critical as when using OPA. [Pg.53]

PITC Amino acid PTC-amino acid Simplified reaction between PITC and amino acids... [Pg.53]

Reaction with phenylisothiocyanate (PITC) in alkaline conditions produces stable phenylthiocarbamyl (PTC) adducts which can be detected either in the ultraviolet below 250 nm or electrochemically. However, this method involves a complex derivatization procedure and offers poorer sensitivity than the alternatives available for individual amino acids. It is useful, however, in conjunction with the automated analysis of peptides when single derivatized residues can be cleaved and analysed after conversion in acidic conditions to phenylthiohydantoins. [Pg.373]

FIGURE 19.5 HPLC profile of PITC derivatives of amino acids from a red seaweed (UV 254nm). (From Sanchez-Machado, D.I. et al., Chromatographia, 58, 159, 2003. With permission from Springer Science + Business Media.)... [Pg.589]

B PITC (phenyhsothiocyanate), undiluted. Sequence Grade C n-heptane/ethylacetate 2 1 (v/v)... [Pg.83]

Figure 24 Incident photon energy dependence of the surface charge separation efficiency (h" / photon) measured by PITCS. Squares, circles, triangles, and diamonds represent the results obtained for Cr-implanted rutile, Cr-doped rutile, undoped rutile, and anatase Ti02 films, respectively. Figure 24 Incident photon energy dependence of the surface charge separation efficiency (h" / photon) measured by PITCS. Squares, circles, triangles, and diamonds represent the results obtained for Cr-implanted rutile, Cr-doped rutile, undoped rutile, and anatase Ti02 films, respectively.

See other pages where PITC is mentioned: [Pg.530]    [Pg.214]    [Pg.1031]    [Pg.1032]    [Pg.56]    [Pg.250]    [Pg.214]    [Pg.166]    [Pg.167]    [Pg.307]    [Pg.327]    [Pg.86]    [Pg.142]    [Pg.53]    [Pg.19]    [Pg.18]    [Pg.26]    [Pg.587]    [Pg.588]    [Pg.591]    [Pg.591]    [Pg.591]    [Pg.591]    [Pg.592]    [Pg.592]    [Pg.592]    [Pg.593]    [Pg.596]    [Pg.643]    [Pg.645]    [Pg.133]    [Pg.231]    [Pg.839]    [Pg.2]    [Pg.3]   


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PITC determination

Phenylisothiocyanate (PITC Edman

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