Pink-eyed mutation

Mouse C57B1/6J jy/p " carrying an autosomal recessive mutation that produces pink eyes. 

Bishop, A. J., Kosaras, B., Sidman, R. L., and Schiestl, R. H. (2000). Benzo(retinal pigment epithelium of mice. Mutat Res 457, 31 0. 

Gondo, Y., Gardner, J. M., Nakatsu, Y., Durham-Pietre, D., Deveau, S. A., Kuper, C., and Brilliant, M. H. (1993). High-frequency genetic reversion mediated by a DNA duplication The mouse pink-eyed unstable mutation. Proc Nad Acad Sci USA 90, 297-301. 

Schiestl, R. H., Aubrecht, J., KhogaU, F., and Carls, N. (1997). Carcinogens induce reversion of the mouse pink-eyed unstable mutation. Proc Natl Acad Sci USA 94, 4576-4581. 

In mutagenesis the main condition of success is the development of a sensitive and rapid method of mutant selection. Vitamin B12 production by propionic acid bacteria correlates with acid production. But the method of selection based on picking colonies with the maximal diameter of dissolution zones of calcium carbonate (added to solid medium) did not give positive results, since the size of colonies is not identical while acid production is dependent on the quantity of biomass. Selection based on the intensity of pink color that correlates with vitamin B12 production has a disadvantage in that selection of small mutations is made step by step, when differences in color can hardly be noticed by eye. Another method used was based on direct determination of vitamin B12 in single colonies. This method is labor-intensive and severely limits the testing of large numbers of colonies. 

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