Physical Parameters for High-speed Separations

It was thought that high-speed separations would be achieved by the development of a physically stable pumping system and highly sensitive detectors  

Comparison of these four chromatograms suggests that a fast separation can be performed either using a longer column with 10 pm stationary phase material with a high flow rate of the eluent, to give high resolution, or by a smaller 

Basic Concepts of High-performance Liquid Chromatography 

This equation indicates that the particle size, dp, is the main contributor to the H value. The smaller the particles, the higher the theoretical plate number. The optimum condition is obtained by the relationship between the theoretical plate height and the flow velocity. 

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