Phycoerythrin conjugate with streptavidin

Naegleria fowleri (Pougnard et al. 2002) has been detected by SPC in surface water. A monoclonal antibody was conjugated with biotin or HRP and revealed by streptavidin conjugated to RPE-Cy5 (R-phycoerythrin conjugated with the cyanine dye Cy5) or FITC-conjngated tyramide, respectively. The RPE-Cy5 protocol was the most efficient and allowed the detection of both trophozoite and cyst forms in water. 

Fig. 5.2. Methods for detection of passenger-ligand interaction. (A) Fluorophore-coupled ligand (B) fluorophore-coupled antibody (C) quaternary complex generated by subsequent rounds of incubation with ligand, primary antibody, biotinylated second antibody, and strep ta-vidin, R-phycoerythrin conjugate (D) quaternary complex generated by subsequent rounds of incubation wi tli ligand, primary antibody, biotinylated second antibody, and streptavidin-coated magnetobeads. L ligand P passenger.

Figu re 4.8 Flow cytometry analysis of silica nanoparticles surface-modified with proteins. MPMS nanoparticles (a, c) and TEOS nanoparticles (b, d) modified with GFP (a, b) or phycoerythrin-conjugated streptavidin... 

The effects of CVS on the numbers of T-cell subsets were examined on day 9 at the time of tumor i.v. rechallenging, Fig. (6)-Protocol B. Cells were stained with fluorescein 5-isothiocyanate (FITC)-, phycoerythrin (PE)-conjugated and/or biotinylated mAb and were additionally stained with RED-613-streptavidin for flow-cytometric three-color analysis. 

---