Photosynthetic HPLC separation

Schoefs, B., Lemoine, Y., and Bertrand, M. 1996. Reversed-phase HPLC separation of photosynthetic pigments and their precursors. Am. Biotechnol. Lab. 14, 18-22. 

Figure 2 Reversed-phase LC separation of deuterated chlorophyll a and undeuterated chlorophyll a on a 25 cm x 4.6 mm i.d. Ci8-Ultrasphere CDS column. Mobile phase water-methanol-acetonitrile-tetrahydrofuran 5 28 38 23 (v/v/v/v). UV detector 663 nm. (Reprinted with permission from Baweja R, et al. (1986) HPLC separation of deuterated photosynthetic pigments from their protio analogues. Journal of Chromatography 369 125-131 Elsevier.)...

Baweja R, et al. (1986) HPLC separation of deuterated photosynthetic pigments from their protio analogues. Journal of Chromatography 369 125-131. 

Photosynthetic pigments were separated and quantified by HPLC (Beckman System-Gold with a 15 cm stainless steel C a column) essentially as described previously (12) except the flow <2 cm .min"" ) and the mobile phase. This phase consisted in two isocratic steps first (6 min) acetonl-trile/ethylacetate (50/50 v/v) and second (10 min) dichloro-... 

The separation of photosynthetic pigments by HPLC was obtained by using the system described in material and methods which permitted the clear resolution of zeaxanthin and lutein (Fig. 1). This method allowed to study the changes produced in photosynthetic pigments, and in particular, those of the xanthophylls involved in the violaxanthin cycle. 

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