Photopolymerized sol-gel monolith

Kato, M., Jin, H. M., Sakai-Kato, K., Toyo oka, T., Dulay, M. T., and Zare, R. N. (2003). Determination of glutamine and serine in rat cerebrospinal fluid using capillary electrochromatography with a modified photopolymerized sol-gel monolithic column. /. Chromatogr. A 1004, 209-215. 

Dulay MT, Quirino JP, Bennett BD, Kato M, Zare RN (2001) Photopolymerized sol-gel monoliths for capillary electrochromatography. Anal Chem 73 3921-3926... 

Trypsin covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) (Scheme 10.13) was described by Dulay and others [126] for on-column digestion of N-a-benzoyl-L-arginine ethyl ester (BAEE) and peptides, neurotensin, and insulin chain B. They prepared a novel IMER by preparation of a reactive hydrophilic macroporous PEG-modified photo-polymerized... 

To ameliorate the problems associated with sintered frits alternative approaches to frit fabrication continue to be developed. Some promising methods include sol-gel-glued packing materials [321,322], thermally polymerized silicate solutions [323], monolithic frits formed by photopolymerization of a mixture of organic monomers and a porogen [293,324] and tapered joints (which allow fritless column configurations) [310,325]. The robustness of these methods remains to be explored. None are simple, but all tackle at least some of the problems associated with sintered frits, and provide a platform for further development. 

It has been possible to fabricate porous monoliths within silicon [16], glass [17], and polymer [18, 19] microchaimels. Within silica and glass channels, porous monoliths can be fabricated by a thermally aided sol-gel reaction. Photopolymerization of acrylate monomers in the presence of porogens (inert organic solvents) has also been used to obtain porous monoliths. By varying the composition of the monomer blend, the composition of the porogen mixture, and the ratio of monomer to porogen, it is possible to control the porosity of the monolith and the modal size of its pores. In addition, if the channels are made out of acrylate... 

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