Photochromic FRET

Photochromic FRET using as acceptors the photochromic compounds such as spiropyrans [36]. They have the ability to undergo a reversible transformation between two different structural forms in response to illumination at appropriate wavelengths. These forms may have different absorption (and in some cases,... 

QDs excited by single or multiple photons exhibit multixponen-tial decays, in our hands generally with two components of <10 and 10-25 ns. This property renders the nanoparticles useful reagents for the systematic modulation of emission via a technique we have denoted as photochromic FRET (pcFRET, Fig. 12.5 see [1, 2, 134]). The method was developed in order to circumvent inherent limitations in the quantitative determination of FRET in cells, particularly in imaging applications. We have classified pcFRET as a member of the family of so-called acceptor depletion methods [1, 2] due to the reversible change in the structure, and thus spectroscopic properties, of the photochromic compound (e.g., a diheteroarylethene) serving as the FRET acceptor. The key... 

The "Free Rotor" or "Loose Bolt" Effect on Quantum Yields 953 Single-Molecule FRET 961 Tni/js-Cyclohexene 967 Retinal and Rhodopsin— The Photochemistry of Vision 968 Photochromism 969... 

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