Photochemical crosslinking

Oster, G., "Photochemical Crosslinking of Non-Aqueous Polymers by Near Ultraviolet Light", J. Polym. Sci., 1964, 2, ... 

Figure 6.9 Photochemical crosslinking of a PET-p-phenylene bisacrylic acid (PBA) copolymer...

Thibault RJ, Uzun O, Hong R, Rotello VM. Recognition-controlled assembly of nanoparticles using photochemically crosslinked recognition-induced pol3miersomes. Adv Mater 2006 18 2179-2183. 

The peptidyltransferase site. The position was located by binding of derivatives of the antibiotic puromycin (Fig. 29-13). An arylazide derivative of puromycin was photochemically linked (Eq. 23-27) to proteins L23, L18/22, and L15 immunoelectron microscopy, using antibodies to the -dimethyl-adenosine of puromycin,165 166 located the binding site adjacent to the central protuberance between the 50S subunit and 30S subunit near S14.5 4-Thio-dT-p-C-p-puromycin was photochemically crosslinked to G2553... 

The demonstration of stepwise adduct formation by psoralens was an important advance. At 390 nm monoadduct formation occurs a crosslink is completed by irradiation at 360 nm (Chatterjee and Cantor, 1978). DNA-psoralen monoadducts have been used to covalently label complementary sites on large RNA molecules. The sites may then be mapped by electron microscopy of the denatured nucleic acid complexes (Wollenzien and Cantor, 1982). Psoralen derivatives for attachment to proteins have been made (Cantor, 1980) and this will allow the exploration of protein binding sites on nucleic acids by photochemical crosslinking. 

It will be noticed that in this, and in all other examples of photochemical crosslinking, that crosslinking is far from complete and it seems that a substantial proportion of the photogenerated intermediates must react with buffer components or water, or form highly unstable bonds. Intramolecular crosslinks may be reflected in spots above the diagonal or worse as streaks in the first dimension (Huang and Richards, 1977). 

The most successful experiments for photolocking have used the technique of attaching the dopant to the base polymer. It is obvious that this is an effective way to fix a small molecule in place and that it should work at low levels of dopant. It is possible to incorporate the ability for photochemical crosslinking into the film at the same time so as to improve the prospects for high resolution. 

Title Photochemical Crosslinkers for Polymer Coatings and Substrate Tie-Layer... 

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