Phosphomono-esterases

Phytase phosphomono- esterase sulfoxidation sulfoxidation aided by vanadate addition van de Velde, 1998... 

Gaur, A. S. Agarwal, S. M. (1981). Non-specific phosphomono-esterases in three species of caryophyllids from Clarias batrachus (Linn.). Proceedings of the Indian Academy of Parasitology, 2 71-5. 

A7. Akamatsu, S., and Kobayashi, K., Chemical nature of the co-phosphomono-esterase. Emymologia 16, 154-157 (1951). 

Sequencing.—The technique of polynucleotide sequencing by continuous directional degradation, in which incubation with alkaline phosphomono-esterase and periodate gives sequential release from the 3 -end of nucleoside dialdehydes, which are reduced with borotritide and identified chromato-graphically, has been refined at micromolar concentrations, and a better... 

Treatment of bromomethyl acetate with the sodium salt of a dialkyl phosphite, followed by deacetylation with methoxide affords the corresponding dialkyl hydroxymethanephosphonate. If this is tosylated with tosyl chloride, and the product treated with nucleosides [protected at the 3 -(and 2 -, if present) hydroxy groups] and sodium hydride in DMF, the monoalkyl ester of a 5 -0-phosphonyl-methyl nucleoside (60) is obtained after deblocking the sugar, and subsequent dealkylation with TMS-iodide affords (61). Like alkyl esters of 5 -mononucleotides, (60) is resistant to acid and alkaline hydrolysis, while (61) is stable in acidic and alkaline media, and is also resistant to hydrolysis by alkaline phosphomono-esterase and snake venom 5 -nucleotidase. Treatment of (61) with DCC and morpholine, and subsequently with orthophosphate or pyrophosphate, affords (62) and (63), respectively. Alkaline phosphomonoesterase from E. co/i hydrolyses the pyrophosphate links in (62) and (63) to give (61) and orthophosphate. The UTP and CTP analogues (63 B = U or C) are inhibitors of uridine kinase from... 

Snake venom phosphodiesterase does not attack dinucleotides in which a free phosphomonoester occurs on a 3 position. Several phosphomono-esterases exist that can remove monoester phosphates from these and, indeed, from RNA. 

Pyrimidine Nvdeoside Diphosphates. When ribonucleic acid is digested with purified snake venom diesterase (free of phosphomono-esterase activity) approximately 20% of the total base can be accounted for as 2, 5 and/or 3, 5 diphosphates of cytidine and uridine. The mechanism of origin of these compounds is discussed below. 

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