Phospholipase incubation conditions

For some foods, incomplete extraction of color is obtained, probably due to the high binding affinity of dyes to the bulk of the food matrix, especially to proteins, lipids, and carbohydrates (156,161,162). This problem can be overcome by the use of selected solvents or enzymes to digest the food prior to extraction. Petroleum ether can be used to extract lipids (163). Acetone can be used to remove lipids and coagulate protein (164). Enzymes, such as amyloglucosidase (165,166), papain (167), lipase, pectinase, cellulase, and phospholipase, added to the sample and incubated under optimum pH and temperature conditions release synthetic colors bound to or associated with the food matrix. Furthermore, enzyme digestion can solubilize some foods, enabling analysis to be continued (156). 

The enzymes which catalyse drug metabolism are not associated with the ribosomes but are found in the membranous vesicles. Accordingly, incubation of micro-somes with ribonuclease has no effect on drug-metabolizing activity whereas conditions which disrupt lipid membranes such as incubation with lipase or phospholipase, exposure to organic solvents or detergents result in enzyme inactivation. 

Thylakoids were incubated in the presence of either the pancreatic phospholipase A2 or the lipase from Rhizopus arrhizus under conditions ( bovine serum albumin, BSA, EGTA, appropriate pH, T cind ionic... 

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