Phosphofructokinase expression

R2. Raben, N., Exelbert, R., Spiegel, R., Sherman, J. B Nakajima, H., Plotz, P., and Heinisch, J., Functional expression of human mutant phosphofructokinase in yeast Genetic defects in French Canadian and Swiss patients with phosphofructokinase deficiency. Am. J. Hum. Genet. 56, 131-141 (1995). 

Ronimus, R.S. de Heus, E. Morgan, H.W. Sequencing, expression, characterization and phylogeny of the ADP-dependent phosphofructokinase from the hyperthermophilic, euryarchaeal Thermococcus zilligii. Biochim. Biophys. Acta, 1517, 384-391 (2001)... 

Most or all ATP-requiring enzymes use ATP in the form of the Mg-ATP complex. ATP chelates the magnesium ion, which is a divalent metal ion. The glycolytic pathway features a number of Mg-requiring enzymes. One of these enzymes is phosphofructokinase. The Mg requirement for this enzyme is illustrated by the data in Figure 10-50- Ihe study involved phosphofructokinase purified from rabbit muscle. Each point in Figure 10-50 represents the catalytic activity of the enzyme that was expressed during incubation in separate test tubes. All of the test tubes... 

This may change the initial velocity pattern, as in the case of fructose-6-sulfate, which is a slow substrate for phosphofructokinase. This substrate has lost sufficient affinity for the enzyme that it binds only when MgATP is present, and thus the mechanism changes from a random one with both substrates sticky with fructose 6-phosphate (fructose-6-P) to an equilibrium ordered one with MgATP adding first (19). Slow alternate substrates give cleaner and more easily interpreted pH profiles, and isotope effects are often (but not always) more fully expressed (see Sections VII,A and VII,B below). 

Bruchhaus, L Jacobs, T. Denart, M. Tannich, E. Pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica molecular cloning, recombinant expression and inhibition by pyrophosphate analogs. Biochem. J., 316, 57-63 (1996)... 

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