Phospho group

If the action of the enzyme is to transfer the phospho group, e.g., to water in a hydrolysis, or intramolecularly, e.g., in converting 3-phosphoglycerate into 2-phosphoglycerate (48), it cannot transform the phosphonomethyl analogue. This is because R—CH2 is an incompara-... 

A remarkable reaction of the phospho group is its transfer onto carbon in the biosynthesis of the C—P bond (Fig. 6). Incidentally, this leads to a difficulty with nomenclature, because the group -P03H2, which is known to chemists as phosphono, is called phospho only when on a heteroatom (64), so the transfer changes its name Despite much ealier guessing from labeling patterns that phosphoenolpyruvate was the source of the C—P bond, it was only in 1988 (65, 66) that the enzyme responsible was isolated. The difficulty proved to be that the equilibrium favors phosphoenolpyruvate by about 2000-fold (67), so that assays only detected the enzyme in the direction contrary to biosynthesis evidently the biosynthesis takes place because subsequent reac-... 

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