Phenol-water micro-extraction

Because LPS molecules in different bacteria have different structures and amphi-pathic properties, there is no universal method for extraction of LPS. Several methods have been developed each favors some specific groups of LPS. For example, the phenol-water extraction method favors S-LPS extraction, but not R-LPS (Hickman and Ashwell, 1966 Kasai and Nowotny, 1967) the ether extraction method favors the extraction of R-LPS (Galanos et al., 1969). Both methods for large scale or micro-scale extractions have been developed. Agents used by different extraction methods are listed in Table 2.1. 

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