Phage ELISA

Take 50-100 pL of phage supernatant for phage ELISA, as described in Section 3.6.1... 

We described here the cloning of Evasin open-reading frames into the pMSlOlC vector, the introduction of alanine mutations into these sequences and the screening of alanine mutants by phage ELISA and soluble ELISA (Figs. 1 and 2). 

Figure 2 Screening of alanine mutants by phage display. Nine alanine mutants of a CKBP were analyzed by phage ELISA to determine which amino acids are involved in the interaction with the chemokine. Based on these results, mutants 1, 3, and 5 display a clearly reduced binding to the chemokine.

A useful sensitive primary screening process for antibodies on the basis of their binding to immobilized antigen is the phage ELISA. Individual colonies generated by the selection proce.ss can be picked into 96-we 11 format and phage mid rescue carried out. The resultant phage particles can then be used directly in an... 

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